2011-17-LF Intraocular lenses with functionalized surfaces by biomolecules in relation with lens epithelial cell adhesion (ULG-MSLAB&LHC, UB1-CBMN)

The most common complication after implantation of intraocular lenses (IOLs) is the posterior capsular opacification (PCO) or secondary cataract [1]. This is the result of lens epithelial cells proliferation and their transition to mesenchymal cells. IOLs are elaborated from polymers of various hydrophobic to hydrophilic character. Their degree of biocompatibility must be very high while maintaining optimal optical properties. The project aims to graft biopolymers (extracellular matrix proteins or fragments) on hydrophobic and hydrophilic IOLs in order to evaluate the resulting interactions with lens epithelial cells and endothelial cells. Complementary investigation techniques and methods will be used to study the surface properties and the biocompatibility of functionalized IOls, in order to define performance indices related to cell adhesion and PCO.

IOLs will be functionalized with active principles (to modulate cell adhesion). Physico-chemical characterization of material surface will be realized. The surface properties will be monitored using contact angle measurements, Atomic Force Microscopy (AFM), Zeta potential, profilometry, x-ray photoelectron spectroscopy (XPS), FTIR/ATR, Scanning Electron Microscopy (SEM).

in vitro of affinity of the active principles will be performed on IOLs by using fluorescent markers.

Adhesion of lens epithelial cell, lens capsule or endothelial cells on IOls will be quantified. Metabolic tests, histological and SEM analyses will be performed to evaluate the cell adhesion, proliferation and differentiation in vitro.

Search for biomarkers of interactions of IOL with surrounding cells using proteomics techniques including MALDI imaging will be conducted on the various models.

In vivo analyses of tissues associated to IOLs by histology and mass spectrometry (IOLs implanted in rabbit model).

[1] D. Bozukova et al. Materials Science and Engineering R 2010, 69, 63 – 83.

Localization and identification of cristallyns on lens slices

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Image: Spatial distribution of lens soluble crystallins by MALDI imaging associated with ISD to sequence and identify proteins Project Partners

The industrial partner (Physiol) will participate in model conception, in designing IOLs and polymers.

Laboratory CBMN from University of Bordeaux will be implicated in the functionalization and characterization of the new IOLs.

Laboratory LHC from University of Liège will be implicated in cell culture models, histology, biocompatibility tests and mass spectrometry.

In vivo biocompatiblity tests will be performed with the complementary competences in Liège and Bordeaux.